MiR-326 regulates cell proliferation and apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis.

The dysregulation of microRNAs plays a critical role in the development of rheumatoid arthritis (RA). This study aims to explore the functional significance of miR-326 in RA. The RT-qPCR results showed that miR-326 was downregulated in synovial tissues of RA patients and RA fibroblast-like synoviocytes (RA-FLS). We found that miR-326 could target and reduce the expression of inhibitor of DNA binding 1 (Id1). MTT assay and flow cytometry were conducted to explore the biological function of miR-326. Our data revealed that the upregulation of miR-326 suppressed cell proliferation and induced apoptosis in RA-FLS. In collagen-induced arthritis mice, intraarticular injection of lentivirus carrying miR-326 overexpression vectors could reduce the arthritis score and attenuate synovial inflammation and cartilage destruction. We also found that long non-coding RNA-Ewing sarcoma-associated transcript 1 (lncRNA-EWSAT1) was significantly increased in RA synovial tissues and RA-FLS. The RNA immunoprecipitation and RNA pull-down assay indicated that lncRNA-EWSAT1 directly bound and negatively regulated the expression of miR-326. Knockdown of lncRNA-EWSAT1 could upregulate miR-326 expression and attenuate its proliferation inhibition and apoptosis induction effect in RA-FLS.  In conclusion, the lncRNA-EWSAT1/miR-326 axis might provide a novel therapeutic target in the treatment of RA.

Postoperative analgesia efficacy of ultrasound-guided rectus abdominis sheath block in patients with abdomen panhysterectomy / 中国医师进修杂志

Objective To investigate the postoperative analgesia efficacy of ultrasound-guided rectus abdominis sheath block (RSB) in patients with abdomen panhysterectomy.Methods Forty patients with abdomen panhysterectomy under general anesthesia were divided into RSB group and control group by random digits table method with 20 cases each.All the patients were given patient controlled intravenous analgesia.The visual analogue scale (VAS) score and Ramsay sedative score at 2,6,12 and 24 h after operation,sulfentanyl dosage and total number of analgesic pump press of 24 h,number of nausea and vomiting,analgesic satisfaction score were recorded.Results The VAS scores in RSB group at 2,6,12,24 h after operation were lower than those in control group,but there were no statistical differences (P＞ 0.05).The Ramsay sedative score of all patients was ＜ 4 scores.Sulfentanyl dosage of 24 h in RSB group was (73.1 ± 8.6) μ g,in control group was (91.7 ± 12.3) μ g,there was statistical difference (P ＜ 0.01).The number of analgesic pump press of 24 in RSB group was (6.5 ± 2.1) times,in control group was (12.3 ± 4.6)times,there was statistical difference (P ＜ 0.01).Analgesic satisfaction score in RSB group was (7.3 ± 0.7)scores,in control group was (6.8 ± 1.1) scores,there was statistical difference (P＜ 0.05).Four cases in RSB happened nausea and vomiting,3 cases in control group,there was no statistical difference (P ＞ 0.05).All patients did not happen the complication related with RSB operation.Conclusion RSB as the postoperative analgesia in patients with abdomen panhysterectomy can reduce the dosage of opioid drug,and increases the analgesic satisfaction.